Recombinant:Article Title: Exploration of Hydrazide-Based HDAC8 PROTACs for the Treatment of Hematological Malignancies and Solid Tumors.
Article Snippet: .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog#: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog#: 50052), human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog#: 50003) and human recombinant C-terminal His-tag HDAC8 (BPS Bioscience, Catalog#: 50008) were diluted in assay buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL BSA), HDAC1(1.87 ng/μL), HDAC2 (1.87 ng/μL), HDAC3 (0.89 ng/μL), and HDAC8 (1.16 ng/μL). ..
Article Title: Design, Multicomponent Synthesis, and Anticancer Activity of a Focused Histone Deacetylase (HDAC) Inhibitor Library with Peptoid-Based Cap Groups.
Article Snippet: 46 Black 96-well flat bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)AMC (20 μM, Bachem, Germany) at 37 °C.
Article Title: Synthesis of Peptoid-Based Class I-Selective Histone Deacetylase Inhibitors with Chemosensitizing Properties
Article Snippet: Black 96-well flat bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG- tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His- tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)- AMC (20 μM, Bachem, Germany) at 37 °C.
Article Title: Induced protein degradation of histone deacetylases 3 (HDAC3) by proteolysis targeting chimera (PROTAC)
Article Snippet: Black 96-well flat-bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog #: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mMMgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 mL of this dilutionwas incubatedwith 10 mL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 mL of the fluorogenic Boc-Lys(ε-Ac)-AMC (20 mM, Bachem, Germany) at 37 C. After 90 min incubation time 50 mL of the stop solution (25 mM Tris-HCl (pH 8), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.01% Triton-X, 6.0 mg/mL trypsin (porcine pancreas Type IX-S, lyophilized powder, 13,000e20,000 BAEE units/mg protein, Sigma Aldrich) and 200 mM vorinostat) was added.
Article Title: Exploration of Hydrazide-Based HDAC8 PROTACs for the Treatment of Hematological Malignancies and Solid Tumors
Article Snippet: .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog#: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog#: 50052), human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog#: 50003) and human recombinant C-terminal His-tag HDAC8 (BPS Bioscience, Catalog#: 50008) were diluted in assay buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , and 0.1 mg/mL BSA), HDAC1(1.87 ng/μL), HDAC2 (1.87 ng/μL), HDAC3 (0.89 ng/μL), and HDAC8 (1.16 ng/μL). ..
Article Title: HDAC/MIF dual inhibitor inhibits NSCLC cell survival and proliferation by blocking the AKT pathway.
Article Snippet: Black 96-well flat-bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog #: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)-AMC (20 mM, Bachem, Germany) at 37 ◦C.
FLAG-tag:Article Title: Exploration of Hydrazide-Based HDAC8 PROTACs for the Treatment of Hematological Malignancies and Solid Tumors.
Article Snippet: .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog#: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog#: 50052), human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog#: 50003) and human recombinant C-terminal His-tag HDAC8 (BPS Bioscience, Catalog#: 50008) were diluted in assay buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL BSA), HDAC1(1.87 ng/μL), HDAC2 (1.87 ng/μL), HDAC3 (0.89 ng/μL), and HDAC8 (1.16 ng/μL). ..
Article Title: Design, Multicomponent Synthesis, and Anticancer Activity of a Focused Histone Deacetylase (HDAC) Inhibitor Library with Peptoid-Based Cap Groups.
Article Snippet: 46 Black 96-well flat bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)AMC (20 μM, Bachem, Germany) at 37 °C.
Article Title: Synthesis of Peptoid-Based Class I-Selective Histone Deacetylase Inhibitors with Chemosensitizing Properties
Article Snippet: Black 96-well flat bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG- tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His- tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)- AMC (20 μM, Bachem, Germany) at 37 °C.
Article Title: Induced protein degradation of histone deacetylases 3 (HDAC3) by proteolysis targeting chimera (PROTAC)
Article Snippet: Black 96-well flat-bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog #: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mMMgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 mL of this dilutionwas incubatedwith 10 mL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 mL of the fluorogenic Boc-Lys(ε-Ac)-AMC (20 mM, Bachem, Germany) at 37 C. After 90 min incubation time 50 mL of the stop solution (25 mM Tris-HCl (pH 8), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.01% Triton-X, 6.0 mg/mL trypsin (porcine pancreas Type IX-S, lyophilized powder, 13,000e20,000 BAEE units/mg protein, Sigma Aldrich) and 200 mM vorinostat) was added.
Article Title: Exploration of Hydrazide-Based HDAC8 PROTACs for the Treatment of Hematological Malignancies and Solid Tumors
Article Snippet: .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog#: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog#: 50052), human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog#: 50003) and human recombinant C-terminal His-tag HDAC8 (BPS Bioscience, Catalog#: 50008) were diluted in assay buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , and 0.1 mg/mL BSA), HDAC1(1.87 ng/μL), HDAC2 (1.87 ng/μL), HDAC3 (0.89 ng/μL), and HDAC8 (1.16 ng/μL). ..
Article Title: HDAC/MIF dual inhibitor inhibits NSCLC cell survival and proliferation by blocking the AKT pathway.
Article Snippet: Black 96-well flat-bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog #: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)-AMC (20 mM, Bachem, Germany) at 37 ◦C.
Incubation:Article Title: Design, Multicomponent Synthesis, and Anticancer Activity of a Focused Histone Deacetylase (HDAC) Inhibitor Library with Peptoid-Based Cap Groups.
Article Snippet: 46 Black 96-well flat bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)AMC (20 μM, Bachem, Germany) at 37 °C.
Article Title: Synthesis of Peptoid-Based Class I-Selective Histone Deacetylase Inhibitors with Chemosensitizing Properties
Article Snippet: Black 96-well flat bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG- tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His- tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)- AMC (20 μM, Bachem, Germany) at 37 °C.
Article Title: Induced protein degradation of histone deacetylases 3 (HDAC3) by proteolysis targeting chimera (PROTAC)
Article Snippet: Black 96-well flat-bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog #: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mMMgCl, 0.01% Triton-X and 1 mg/mL BSA). .. 40 mL of this dilutionwas incubatedwith 10 mL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 mL of the fluorogenic Boc-Lys(ε-Ac)-AMC (20 mM, Bachem, Germany) at 37 C. After 90 min incubation time 50 mL of the stop solution (25 mM Tris-HCl (pH 8), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.01% Triton-X, 6.0 mg/mL trypsin (porcine pancreas Type IX-S, lyophilized powder, 13,000e20,000 BAEE units/mg protein, Sigma Aldrich) and 200 mM vorinostat) was added.
Article Title: HDAC/MIF dual inhibitor inhibits NSCLC cell survival and proliferation by blocking the AKT pathway.
Article Snippet: Black 96-well flat-bottom microplates (Corning® Costar®, Corning Incorporated, NY) were used. .. Human recombinant C-terminal FLAG-tag, C-terminal His-tag HDAC1 (BPS Bioscience, Catalog #: 50051), Human recombinant C-terminal FLAG-tag HDAC2 (BPS Bioscience, Catalog #: 50052) or human recombinant C-terminal His-tag HDAC3/NcoR2 (BPS Bioscience, Catalog #: 50003) were diluted in incubation buffer (25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.01% Triton-X and 1 mg/mL BSA). .. 40 μL of this dilution was incubated with 10 μL of different concentrations of inhibitors in 10% DMSO/incubation buffer and 50 μL of the fluorogenic Boc-Lys(ε-Ac)-AMC (20 mM, Bachem, Germany) at 37 ◦C.
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